GATA3 antibody (AA 175-388)

Catalog No. ABIN969167

The Mouse Monoclonal anti-GATA3 antibody has been validated for ELISA. It is suitable to detect GATA3 in samples from Human. There are 4+ publications available.

Quick Overview for GATA3 antibody (AA 175-388) (ABIN969167)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GATA3 antibody is un-conjugated

Application: ELISA

Clone: 1A10D1

Product Details anti-GATA3 Antibody

Binding Specificity: AA 175-388

Purpose: GATA3 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of GATA3 (aa175-388) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-GATA3 antibody (ABIN969167)

Gulbinas, Berberat, Dambrauskas, Giese, Giese, Autschbach, Kleeff, Meuer, Büchler, Friess: "Aberrant gata-3 expression in human pancreatic cancer." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 2, pp. 161-9, (2006) (PubMed).

Erpenbeck, Hagenberg, Krentel, Discher, Braun, Hohlfeld, Krug: "Regulation of GATA-3, c-maf and T-bet mRNA expression in bronchoalveolar lavage cells and bronchial biopsies after segmental allergen challenge." in: International archives of allergy and immunology, Vol. 139, Issue 4, pp. 306-16, (2006) (PubMed).

Mehra, Varambally, Ding, Shen, Sabel, Ghosh, Chinnaiyan, Kleer: "Identification of GATA3 as a breast cancer prognostic marker by global gene expression meta-analysis." in: Cancer research, Vol. 65, Issue 24, pp. 11259-64, (2005) (PubMed).

Jiang: "Development of a new biotic index to assess freshwater pollution." in: Environmental pollution (Barking, Essex : 1987), Vol. 139, Issue 2, pp. 306-17, (2005) (PubMed).

Target Details for GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: GATA3

Background: GATA3: GATA binding protein 3. The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells, macrophages, or HeLa cell lines. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1,but no other structural homologies between the 2 genes could be found.

Gene ID: 2625

UniProt: P23771

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation