USP7 antibody

Catalog No. ABIN969186

This anti-USP7 antibody is a Mouse Monoclonal antibody detecting USP7 in WB and ELISA. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for USP7 antibody (ABIN969186)

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This USP7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 5F11

Product Details anti-USP7 Antibody

Purpose: HAUSP Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human HAUSP expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-USP7 antibody (ABIN969186)

Becker, Marchenko, Maurice, Moll: "Hyperubiquitylation of wild-type p53 contributes to cytoplasmic sequestration in neuroblastoma." in: Cell death and differentiation, Vol. 14, Issue 7, pp. 1350-60, (2007) (PubMed).

Lu, Ma, Nguyen, Jones, Oren, Donehower: "The Wip1 Phosphatase acts as a gatekeeper in the p53-Mdm2 autoregulatory loop." in: Cancer cell, Vol. 12, Issue 4, pp. 342-54, (2007) (PubMed).

Target Details for USP7

Target: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Alternative Name: HAUSP

Background: USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP acitivity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.

Molecular Weight: 128 kDa

Gene ID: 7874

UniProt: Q93009