Nanog antibody (AA 20-166)

Catalog No. ABIN969308

The Mouse Monoclonal anti-Nanog antibody has been validated for WB, ELISA and ICC. It is suitable to detect Nanog in samples from Human. There is 1 publication available.

Quick Overview for Nanog antibody (AA 20-166) (ABIN969308)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: 1E6C4

Product Details anti-Nanog Antibody

Binding Specificity: AA 20-166

Purpose: NANOG Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Nanog antibody (ABIN969308)

Freberg, Dahl, Timoskainen, Collas: "Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract." in: Molecular biology of the cell, Vol. 18, Issue 5, pp. 1543-53, (2007) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52 % overall amino acid identity with the mouse protein and 85 % identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.

Molecular Weight: 35 kDa

Gene ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance