ALPL antibody

Catalog No. ABIN969500

The Mouse Monoclonal anti-ALPL antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect ALPL in samples from Human. There are 2+ publications available.

Quick Overview for ALPL antibody (ABIN969500)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 2F4

Product Details anti-ALPL Antibody

Purpose: ALPL Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human ALPL expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-ALPL antibody (ABIN969500)

Kannampuzha, Tupy, Pritzker: "Mercaptopyruvate inhibits tissue-nonspecific alkaline phosphatase and calcium pyrophosphate dihydrate crystal dissolution." in: The Journal of rheumatology, Vol. 36, Issue 12, pp. 2758-65, (2009) (PubMed).

Kanazawa, Yamaguchi, Yamamoto, Yamauchi, Yano, Sugimoto: "Serum osteocalcin/bone-specific alkaline phosphatase ratio is a predictor for the presence of vertebral fractures in men with type 2 diabetes." in: Calcified tissue international, Vol. 85, Issue 3, pp. 228-34, (2009) (PubMed).

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: ALPL

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.

Molecular Weight: 57.3 kDa

Gene ID: 249

UniProt: P05186