Surfactant Protein A1 antibody (AA 102-115)

Catalog No. ABIN987275

The Goat Polyclonal anti-Surfactant Protein A1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Surfactant Protein A1 in samples from Human.

Quick Overview for Surfactant Protein A1 antibody (AA 102-115) (ABIN987275)

Target: Surfactant Protein A1 (SFTPA1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Surfactant Protein A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Surfactant Protein A1 Antibody

Binding Specificity: AA 102-115

Purpose: SFTPA1 / SFTPA2 (aa102-115)

Sequence: HLDEELQATL HDFR

Specificity: This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3, NP_001087239.2, NP_001158117.1, NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HLDEELQATLHDFR, from the internal region of the protein sequence according to NP_005402.3, NP_001087239.2, NP_001158117.1, NP_001158118.1, NP_001092138.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Lung. Recommended concentration: 5 μg/mL.

Western Blot: Approx 30 kDa band observed in Human Lung lysates (calculated MW of 27.7 kDa according to NP_001087239.2). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Surfactant Protein A1

Target: Surfactant Protein A1 (SFTPA1)

Alternative Name: SFTPA1

Background: SFTPA1, surfactant protein A1, COLEC4, FLJ50593, FLJ51913, FLJ61144, FLJ77898, FLJ79095, FLJ99559, MGC133365, MGC198590, PSAP, PSP-A, PSPA, SFTP1, SFTPA1B, SP-A, SP-A1, SPA, SPA1, 35 kDa pulmonary surfactant-associated protein, OTTHUMP00000019928, OTTHUMP

Gene ID: 653509, 729238

NCBI Accession: NP_005402, NP_001087239, NP_001158117, NP_001158118, NP_001092138