Fructose-6-Phosphate Kinase antibody

Details for Product No. ABIN99443
Antigen
Reactivity
Rabbit
13
10
7
7
Host
Goat
13
6
4
Clonality
Polyclonal
Conjugate
Un-conjugated
5
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
18
11
6
5
2
1
1
1
Options
Immunogen Fructose-6-Phosphate Kinase [Rabbit Muscle]
Immunogentype:Native
Characteristics Concentration Definition: by Refractometry
Antigen
Background Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation.  This is a unidirectional and rate-limiting step in glycolysis.  Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate.  The enzyme exists as a homotetramer.
Synonyms: 6 Phosphofructokinase Muscle Type antibody, EC 2.7.1.11 antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody
Gene ID 100345647, 125128
UniProt P00511
Application Notes This purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract. 
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 90 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Nakamura, Mori, Eddy: "Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5." in: Biology of reproduction, Vol. 82, Issue 3, pp. 504-15, 2010 (PubMed).