PFKM antibody

Catalog No. ABIN99443

Product Details anti-PFKM Antibody

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Characteristics: Concentration Definition: by Refractometry

Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]
Immunogentype:Native

Application Details

Application Notes: This purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract. 

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-PFKM antibody (ABIN99443)

Nakamura, Mori, Eddy: "Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5." in: Biology of reproduction, Vol. 82, Issue 3, pp. 504-15, (2010) (PubMed).

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: Fructose-6-Phosphate Kinase (PFKM Products)

Synonyms: GSD7 antibody, PFK-1 antibody, PFK1 antibody, PFKA antibody, PFKX antibody, AI131669 antibody, PFK-A antibody, PFK-M antibody, Pfk-4 antibody, Pfk4 antibody, Pfka antibody, Pfkx antibody, Pfk-M antibody, M-PFK antibody, PFKM antibody, pfkm antibody, zgc:92344 antibody, pfk antibody, im:7137110 antibody, wu:fc58g11 antibody, phosphofructokinase, muscle antibody, phosphofructokinase, platelet antibody, phosphofructokinase, muscle a antibody, phosphofructokinase, muscle S homeolog antibody, phosphofructokinase, muscle b antibody, PFKM antibody, Pfkm antibody, PFKP antibody, pfkma antibody, pfkm.S antibody, pfkmb antibody

Background: Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation.  This is a unidirectional and rate-limiting step in glycolysis.  Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate.  The enzyme exists as a homotetramer.
Synonyms: 6 Phosphofructokinase Muscle Type antibody, EC 2.7.1.11 antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody

Gene ID: 100345647, 125128

UniProt: P00511

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect