PFKM antibody (N-Term)

Catalog No. ABIN2854614

Product Details anti-PFKM Antibody

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to PFK (muscle) (phosphofructokinase, muscle)
PFK (muscle) antibody [N1N2-2], N-term

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Fructose 6 Phosphate Kinase. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse brain , rat brain , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.52 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: phosphofructokinase, muscle (PFKM Products)

Synonyms: GSD7 antibody, PFK-1 antibody, PFK1 antibody, PFKA antibody, PFKX antibody, AI131669 antibody, PFK-A antibody, PFK-M antibody, Pfk-4 antibody, Pfk4 antibody, Pfka antibody, Pfkx antibody, Pfk-M antibody, M-PFK antibody, PFKM antibody, pfkm antibody, zgc:92344 antibody, pfk antibody, im:7137110 antibody, wu:fc58g11 antibody, phosphofructokinase, muscle antibody, phosphofructokinase, platelet antibody, phosphofructokinase, muscle a antibody, phosphofructokinase, muscle S homeolog antibody, phosphofructokinase, muscle b antibody, PFKM antibody, Pfkm antibody, PFKP antibody, pfkma antibody, pfkm.S antibody, pfkmb antibody

Background: The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL, MIM 171860), and platelet (PFKP, MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721], Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]

Molecular Weight: 85 kDa

Gene ID: 5213

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect