PFKM antibody (N-Term)

Catalog No. ABIN392748

The Rabbit Polyclonal anti-PFKM antibody (Clone RB3905) (ABIN392748) specifically detects PFKM in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for PFKM antibody (N-Term) (ABIN392748)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB3905

Product Details anti-PFKM Antibody

Binding Specificity: AA 122-151, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Fructose 6 Phosphate Kinase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the N-terminal region of human Fructose 6 Phosphate Kinase.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: Fructose 6 Phosphate Kinase (PFKM)

Background: Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.

Molecular Weight: 85183

Gene ID: 5213

NCBI Accession: NP_000280, NP_001160158, NP_001160159, NP_001160160

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect