GLUD1 antibody

Catalog No. ABIN99812

Supplier Product No.: 200-4158-0100

This Rabbit Polyclonal antibody specifically detects GLUD1 in WB, IP and ELISA. It exhibits reactivity toward Cow.

Quick Overview for GLUD1 antibody (ABIN99812)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-GLUD1 Antibody

Supplier Product No.: 200-4158-0100

Supplier: Rockland

Purpose: Glutamate Dehydrogenase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver].

Characteristics: Synonyms: rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1

Purification: Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glutamate Dehydrogenase [Bovine Liver]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Glutamate Dehydrogenase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Glutamate Dehydrogenase [Bovine Liver] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:20,000 is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: GLUD1

Background: Background: Glutamate Dehydrogenase, mitochondrial, converts L-glutamate into alpha-ketoglutarate. It plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, which is an important intermediate in the tricarboxylic acid cycle. It may be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. It is subject to allosteric regulation. It is activated by ADP, inhibited by GTP and ATP, and ADP can occupy the NADH binding site and activate the enzyme.

Gene ID: 281785, 32880221

UniProt: P00366

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect