Glycerol Kinase antibody (Biotin)

Catalog No. ABIN99893

Supplier Product No.: 200-106-211-0100

This Goat Polyclonal antibody specifically detects Glycerol Kinase in ELISA and WB. It exhibits reactivity toward Cellulomonas.

Quick Overview for Glycerol Kinase antibody (Biotin) (ABIN99893)

Target: Glycerol Kinase (GK)

Reactivity: Cellulomonas

Host: Goat

Clonality: Polyclonal

Conjugate: This Glycerol Kinase antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Product Details anti-Glycerol Kinase Antibody (Biotin)

Supplier Product No.: 200-106-211-0100

Supplier: Rockland

Purpose: Glycerol Kinase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species].

Characteristics: Synonyms: goat anti-Glycerol Kinase Antibody biotin Conjugation, biotin Conjugated goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody

Purification: Anti-Glycerol Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glycerol Kinase [Cellulomonas species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Glycerol Kinase has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Glycerol Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Glycerol Kinase

Target: Glycerol Kinase (GK)

Alternative Name: glpK

Background: Background: Anti-Glycerol Kinase antibody recognizes the phosphotransferase enzyme glycerol kinase. Glycerol kinase catalyzes the formation of glycerol phosphate by transferring a phosphate from ATP to glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors in place of glycerol. Anti-Glycerol Kinase is ideal for investigators interested in Cancer, Metabolism, and Kinase/Phosphatase enzymology.

Gene ID: 6960048

UniProt: D0VZG4