IP Buffer w/ Triton X-100
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- Application
- Immunoprecipitation (IP)
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
RIPA Lysis BufferIP, WB 10 X
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- Application Notes
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Immunoprecipitation Procedures: Synopsis
Lyse cells
Centrifuge down debris, collect supernatant
Add immunoprecipitating antibody to supernatant
Add immunoprecipitating beads
Gently sediment and wash immunoprecipitating beads
Elute the antibody-protein complex from the immunoprecipitating beads - Comment
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This buffer is applicable to most standard immunoprecipitation procedures. It is first used to lyse cells, followed by the immunoprecipitation of assembled biomolecules. The addition of antibody for immunoprecipitation, subsequent addition of immunoprecipitation beads, such as G-Sepharose, pull down and elution is the general strategy used to reveal direct or in-direct interactions between proteins using this reagent. Triton abrogates hydrophobic interactions, but preserves charge-charge and hydrogen bonding interactions, which are captured and preserved in the immunoprecipitation proceudre. Activity assays or Western blotting analysis are subsequently employed to detect the assembled biomolecular complex.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- RT
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