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Human EIF2C1 Protein expressed in Baculovirus infected Insect Cells - ABIN2004127
Koesters, Adams, Betts, Moos, Schmid, Siermann, Hassam, Weitz, Lichter, Heitz, von Knebel Doeberitz, Briner: Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression. in Genomics 1999
Show all 3 Pubmed References
We chose Drosophila as a model system to take advantage of the presence of two biochemically distinct Argonautes, AGO1 and AGO2. Our results revealed AGO2 loading to be strongly favored by G-rich sequences. In contrast, AGO1 showed an enrichment of the 'GAC' motif in loaded species.
Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies.
findings propose that Ago-1 acts as a key regulator in controlling cell death, tumor regression and stress response in metazoan providing a constructive bridge between RNAi machinery and cell death
Results suggest that Wisp interacts with Ago1 and induces miRNA adenylation and facilitates its downregulation.
Results have established a novel role of Ago-1 as a regulator of the cell cycle.
AGO1 protein level is reduced in the absence of miRNA biogenesis & accumulates on depletion of GW182. Ectopic transcription of miRNAs within in vivo clones & genetic interference with the ubiquitin-proteasome system induced accumulation of AGO1.
Smaug directly recruits Ago1 to nanos mRNA in a miRNA-independent manner, thereby repressing translation.
analysis of regulation of Argonaute slicer activity by guide RNA 3' end interactions with the N-terminal lobe
The study provides a molecular explanation for the previously reported heterogeneity of miRNA 3' ends and proposes a model in which Nibbler converts miRNAs into isoforms that are compatible with the preferred length of Ago1-bound small RNAs.
propose Drosophila Ago-1 as a multifunctional RNAi component that interconnects at least two unrelated events, chromatin organization in the nucleus and microRNA processing in the cytoplasm, which may be extended to the other systems
these results suggest that Ago1 and its miRNA biogenesis partners play a role in oocyte determination and germline cell division in Drosophila.
Ago1-RISC induces silencing via two independent pathways: shortening of the poly(A) tail and pure repression of translation
Mutation of AGO2 or piwi increases silencing at piRNA clusters corresponding to an increase of HP1 association.
Drosophila small RNAs are sorted between Ago1 and Ago2 according to their duplex structure and the identity of their first nucleotide.
nuclear magnetic resonance solution structure of the PAZ domain from Drosophila melanogaster Argonaute 1 (Ago1)
Argonaute1 (AGO1 is required for mature miRNA production that impacts on miRNA-directed RNA cleavage.
Depletion of AGO1, the essential factor for mRNA biogenesis,led to an increased transcriptional rate of the transgenes.
A genome-wide analysis of mRNAs regulated by AGO1 was performed.
Findings argue against a strict separation of Ago1 and Ago2 functions and suggest that these proteins act in concert to control key steps of the midblastula transition and of segmental patterning.
The two members of the Drosophila Ago subclade of Argonaute proteins are functionally specialized, but specific small RNA classes are not restricted to associate with Ago1 or Ago2.
Ago1,Ago2,Ago3 and Piwi are upregulated upon infection with nucleopolyhedrovirus.
Results suggest the two conserved amino acids in the MC motif of BmAgo1 are prerequisites for mRNA translation repression in the silkworm B. mori.
Data show that Ago4- Ago1-Ago3 genes are linked together at the p12 of the chromosome 6, while Ago2 is located at the p15 of the chromosome 4.
AGO1 may promote hepatocellular carcinoma metastasis through TGF-beta pathway.
the polymorphisms of the AGO1 and AGO2 genes, the expression levels of which correlated with the proportion of Th17 cells, were associated with the development and prognosis of Graves' disease.
AGO1 missense mutation was identified in a patient with syndromic form of intellectual disability/autism spectrum disorder.
Effects of the PIWI/MID domain of Argonaute protein on the association of miRNAi's seed base with the target have been reported.
Long A-T repeats up-regulated many genes in cancer that can be targeted by AGO1 to change the expression of many genes and limited cancer growth.
E-cadherin silencing relies on the formation of a complex between the paRNA and microRNA-guided Argonaute 1 that, together, recruit SUV39H1 and induce repressive chromatin modifications in the gene promoter
In miRNA-mediated gene silencing, the physical interaction between human Argonaute (hAgo) and GW182 (hGW182) is essential for facilitating the downstream silencing of the targeted mRNA. hGW182 can recruit up to three copies of hAgo via its three GW motifs. This may explain the observed cooperativity in miRNA-mediated gene silencing.
We selected five single nucleotide polymorphisms (SNPs) (rs7813, rs2740349, rs2291778, rs910924, rs595961) in two key microRNA biosynthesis genes (GEMIN4 and AGO1) and systematically evaluated the association between these SNPs, the gene-environment interaction and lung cancer risk. This is the first study showing that rs7813 and rs595961 could be meaningful as genetic markers for lung cancer risk.
Low AGO1 expression is associated with melanoma.
Blocking AGO1, AGO2, or TRBP expression changes expression levels and nuclear distribution of RNAi factors Dicer, TNRC6A (GW182), and TRBP.
EIF2C2, Dicer, and Drosha are more highly expressed in bladder carcinoma, promote the development of bladder cancer, and suggested a poor prognosis
Argonaute-1 binds transcriptional enhancers and controls constitutive and alternative splicing
Completion of the tetrad, combined with a mutation on a loop adjacent to the active site of hAgo1, results in slicer activity that is substantially enhanced by swapping in the N domain of hAgo2.
Evolutionary amino acid changes to hAGO1 were readily reversible, suggesting that loading of guide RNA and pairing of seed-based miRNA and target RNA constrain its sequence drift.
nuclear Ago1 directly interacts with RNA Polymerase II and is widely associated with chromosomal loci throughout the genome with preferential enrichment in promoters of transcriptionally active genes
Aberrant expression of argonaute-1/-2 in human renal cell carcinoma is possibly involved with tumorigenesis and prognosis.
PIWI-domain mutations in Ago1 may misarrange the catalytic center. Replacing Ago1 cluster 2 by the sequence found in Ago2 fully activated the Ago1 PIWI domain only when the Ago1 PIWI domain was placed into the Ago2 backbone.
The Ago1 N domain performed best when juxtaposed with cognate PAZ and MID. This exemplifies the importance of proper intermolecular-domain interactions.
Study observed a dramatic difference in AGO1 and AGO2 associated miRNA profiles in blood plasma. The lack of correlation between AGO1 and AGO2 miRNA content in the plasma can be explained by the fact that many tissues contribute to the extracellular miRNA content.
Tumor xenografts and human cancer specimens indicate that AGO1-mediated translational desuppression of VEGF may be associated with tumor angiogenesis and poor prognosis.
up-regulated in murine macrophage RAW264.7 cells transfected by Echinococcus multilocularis miR-71
Results from the liver show that, siRNA targets 3'UTR and the coding sequence (CDs) of endogenous genes in the presence Ago2 but in its absence, only 3'UTR-targeted siRNA-mediated knockdown are active with the help of Ago1 and Ago3.
enhanced flu susceptibility of Ago1/3 double-knockout mice arises from an intrinsic impairment in the ability of lung cells to tolerate flu-elicited inflammation.
Ago3 is able to load microRNAs efficiently in the absence of Ago1 and Ago2, despite a significant loss of global microRNA expression
describes cloning rat sequence and used RNA interference to show that the GERp95 orthologue in C. elegans is important for maturation of germ-line stem cells in the gonad.
We show that canonical miRNAs predominantly associate with a high molecular weight (HMW) RISC which co-migrates with AGO1. By providing HTS data we show that the 24-nt siRNAs are mainly associated with a low molecular weight (LMW) RISC co-migrating with AGO4
AGO1 role in the repair of UV-induced DNA lesions
Here is described the identification of AGO1 association with polysomes through polysome fractionation on sucrose gradient, preparation of proteins by filtration and concentration, and immunoblotting.
Results indicate that TRN1 positively regulates miRNA activity by promoting the association of miRNAs with AGO1, and they reveal opposing roles of two importin beta family proteins in miRNA loading.
Sweet potato mild mottle virus P1 interacts with AGO1 and AGO2, but solely interferes with AGO1 function.
Results suggests that ARGONAUTE1 (AGO1) interacts with chromatin at microRNAs MIR161 and MIR173 loci and causes the disassembly of the transcriptional complex, releasing short and unpolyadenylated transcripts.
that AGO1 may have functions in gene regulation independent of small RNAs.
AGO1 is a major, largely HSP90-independent, factor in providing environmental robustness to plants.
miR168 is a key miRNA because it regulates the expression of the slicer protein ARGONAUTE1 (AGO1), which catalyzes mRNA cleavage. Interestingly, plant miR168s are highly evolutionarily conserved; however, it is unclear whether MIR168 promoter elements and expression patterns are also conserved.
a total of 5,123 and 1,399 AGO1-enriched small RNAs, excluding miRNAs, were identified in Arabidopsis thaliana and rice (Oryza sativa), respectively.
Mungbean yellow mosaic Indian virus AC2 interacts with AGO1 and inhibits its transcript slicing activity in vitro.
AGO1, AGO2 and AGO10 promoted anti-TuMV defense in a modular way in various organs, with AGO2 providing a prominent antiviral role in leaves. AGO5, AGO7 and AGO10 had minor effects in leaves.
HOS1 regulates miR168a/b and AGO1 levels in Arabidopsis by maintaining proper transcription of MIR168b.
Base pairing at the 15th nucleotide of a miRNA duplex is important for miRNA sorting in both Arabidopsis AGO1 and AGO2. AGO1 tolerates, or prefers, duplexes with central mismatches.
In the presence of the wild-type allele of AGO1, most of the mas mutations cause a mild or no mutant phenotype on their own, indicating that the ago1-52 mutant may provide a sensitized background for examining the interactions of AGO1.
The results identify AGO1 as a repressor of TFL1 expression. AGO1 plays a role in inflorescence development, controlling the production of coflorescences. AGO1 seems to play this role through regulating TFL1 expression.
AGO1 is required to restrict AGL16-mediated stomatal spacing divisions, an miRNA pathway in addition to ligand-receptor signaling modules.
methylation is required to protect miRNAs from AGO1-associated HESO1 activity that normally uridylates 5' fragments.
Data indicate that the ago1 mutation in hen1 suppressed micriRNA 3' modifications.
Complementation analyses in ago mutant plants revealed that the catalytic residues of AGO1, AGO2, and AGO7 are required to restore the defects of Arabidopsis ago1-25, ago2-1, and zip-1 (AGO7-defective) mutants, respectively.
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4.
, argonaute 1
, argonaute protein 1
, mRNA-like ncRNA in embryogenesis 20
, eukaryotic translation initiation factor 2C, 1
, protein argonaute-1-like
, Golgi Endoplasmic Reticulum protein 95 kDa
, eIF-2C 1
, eIF2C 1
, protein argonaute-1
, putative RNA-binding protein Q99
, Piwi/Argonaute family protein meIF2C1
, argonaute RISC catalytic component 1