FeSOD antibody
This Rabbit Polyclonal antibody specifically detects FeSOD in WB. It exhibits reactivity toward Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa and Zea mays and has been mentioned in 2+ publications.
1 image
(2 references)
Catalog No. ABIN2749232
Quick Overview for FeSOD antibody (ABIN2749232)
Target
FeSOD
(Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))
Reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa, Zea mays
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- No cross-reactivity with: cyanobacteria
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 25 / 22 kDa
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Purification
- serum
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Immunogen
- overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece
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Application Notes
- Recommended Dilution: 1 : 1500 to 1 : 5000 with alkaline phosphatase (WB).
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Comment
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The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 µM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 µL of sterile water.
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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: "Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii." in: The Plant cell, Vol. 24, Issue 6, pp. 2649-65, (2012) (PubMed).
: "Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts." in: The Plant cell, Vol. 17, Issue 4, pp. 1233-51, (2005) (PubMed).
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: "Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii." in: The Plant cell, Vol. 24, Issue 6, pp. 2649-65, (2012) (PubMed).
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- FeSOD (Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))
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Background
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AGI Code: At4g25100
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD -
Molecular Weight
- expected: 25 kDa, apparent: 22 kDa
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UniProt
- A8IGH1
Target
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