Gasdermin A3 Protein (GSDMA3) (AA 1-464) (His tag)
custom-made
GSDMA3
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target
- Gasdermin A3 (GSDMA3)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-464
- Origin
- Mouse
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Gasdermin A3 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Gsdma3 Protein expressed in mammalian cells.
- Sequence
- MPVFEDVTRA LVRELNPRGD LTPLDSLIDF KHFRPFCLVL RKRKSTLFWG ARYVRTDYTL LDLLEPGSSP SDLTDSGNFS FKNMLDVQVQ GLVEVPKTVK VKGTAGLSQS STLEVQTLSV APSALENLKK ERKLSADHSF LNEMRYHEKN LYVVMEAVEA KQEVTVEQTG NANAIFSLPS LALLGLQGSL NNNKAVTIPK GCVLAYRVRL LRVFLFNLWD IPYICNDSMQ TFPKIRRVPC SAFISPTQMI SEEPEEEKLI GEMHEDFKTL KEEVQRETQE VEKLSPVGRS SLLTSLSHLL GKKKELQDLE QKLEGALDKG QKVTLEALPK DVLLSKDAMD AILYFLGALT ELTEEQLKIL VKSLEKKILP VQLKLVESTL EQNFLQDKEG VFPLQPDLLS SLGEEELTLT EALVGLSGLE VQRSGPQYAW DPDTRHNLCA LYAGLSLLHL LSRKSNALTY CALS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Gasdermin A3 (GSDMA3)
- Alternative Name
- Gsdma3
- Background
- Gasdermin-A3 (Gasdermin-3) [Cleaved into: Gasdermin-A3, N-terminal (GSDMA3-NT), Gasdermin-A3, C-terminal (GSDMA3-CT)],FUNCTION: [Gasdermin-A3]: Precursor of a pore-forming protein involved in the transition from catagen to telogen at the end of hair follicle morphogenesis (PubMed:15475261, PubMed:26375003, PubMed:27281216). This form constitutes the precursor of the pore: upon cleavage, the released N-terminal moiety (Gasdermin-A3, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:26375003, PubMed:27281216, PubMed:35545613). This form acts as a sensor of infection: activation is triggered by cleavage by some bacterial effector protein, which releases the N-terminal moiety (Gasdermin-A3, N-terminal) (By similarity). {ECO:0000250|UniProtKB:Q9EST1, ECO:0000269|PubMed:15475261, ECO:0000269|PubMed:26375003, ECO:0000269|PubMed:27281216, ECO:0000269|PubMed:35545613}., FUNCTION: [Gasdermin-A3, N-terminal]: Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed:26375003, PubMed:27281216, PubMed:35545613). Released upon cleavage by some bacterial effector protein, and binds to membrane inner leaflet lipids (By similarity). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukin-1 (IL1B and IL18) and triggering pyroptosis (PubMed:27281216, PubMed:33883744, PubMed:35545613). Binds to membrane inner leaflet lipids, including bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to monophosphorylated phosphatidylinositols (PubMed:27281216). Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216, PubMed:29695864). Plays a role in the transition from catagen to telogen at the end of hair follicle morphogenesis, possibly by regulating hair follicle stem cell niche maintenance (PubMed:15475261, PubMed:15737203, PubMed:17572385, PubMed:22155111, PubMed:32302611). Also required for mammary gland development (PubMed:28168650). {ECO:0000250|UniProtKB:Q9EST1, ECO:0000269|PubMed:15475261, ECO:0000269|PubMed:15737203, ECO:0000269|PubMed:17572385, ECO:0000269|PubMed:22155111, ECO:0000269|PubMed:26375003, ECO:0000269|PubMed:27281216, ECO:0000269|PubMed:28168650, ECO:0000269|PubMed:29695864, ECO:0000269|PubMed:32302611, ECO:0000269|PubMed:33883744, ECO:0000269|PubMed:35545613}.
- Molecular Weight
- 52.0 kDa
- UniProt
- Q5Y4Y6
- AlphaFold
- Q5Y4Y6
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