HSP21 antibody
This Rabbit Polyclonal antibody specifically detects HSP21 in WB. It exhibits reactivity toward Arabidopsis thaliana and Nicotiana tabacum and has been mentioned in 2+ publications.
(2 references)Quick Overview for HSP21 antibody (ABIN3155734)
Target
HSP21
(Heat Shock Protein 21 (HSP21))
Reactivity
Arabidopsis thaliana, Nicotiana tabacum
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- Not reactive in: Chlamydomonas reinhardtii
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Predicted Reactivity
- Arabidopsis thaliana
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 25 / 21 kDa
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Purification
- serum
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Immunogen
- Recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana hsp21 P31170, At4g27670
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Application Notes
- 1: 3000 with standard ECL (WB)
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Comment
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Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water.
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis." in: Plant physiology, Vol. 152, Issue 3, pp. 1471-83, (2010) (PubMed).
: "Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves." in: BMC plant biology, Vol. 10, pp. 34, (2010) (PubMed).
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: "The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis." in: Plant physiology, Vol. 152, Issue 3, pp. 1471-83, (2010) (PubMed).
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- HSP21 (Heat Shock Protein 21 (HSP21))
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Alternative Name
- HSP21
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Background
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AGI Code: At4g27670
Hsp21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. -
Molecular Weight
- expected: 25 kDa, apparent: 21 kDa
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UniProt
- P31170
Target
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