Quick Overview for HEK-293 Cells IgG3 Isotype Control (ABIN2181279)
Target
Application
Isotype
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Reactivity
- Human
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Host
- HEK-293 Cells
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Characteristics
- Human IgG3-Fc / IGHG3 Region has a calculated MW of 31.2 kDa. The predicted N-terminus is Glu 99. The reducing (R) protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.
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Purity
- >95 % as determined by SDS-PAGE.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE
Mouse IgG3 isotype control (FITC)FACS, ELISA Monoclonal B10 IgG3 FITC
Mouse IgG3 isotype control (APC)FACS, ELISA Monoclonal B10 IgG3 APC
Mouse IgG3 isotype control (SPRD)FACS, ELISA Monoclonal B10 IgG3 SPRD
Mouse IgG3 isotype control (Biotin)FACS, ELISA Monoclonal B10 IgG3 Biotin
Mouse IgG3 isotype control (PE-Cy7)FACS, ELISA Monoclonal B10 IgG3 PE-Cy7
Mouse IgG3 isotype controlFACS, ELISA Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype controlFACS, ELISA, IsoC, IHC Monoclonal B10 IgG3 unconjugated
Human IgG3 isotype controlIsoC Polyclonal IgG3 unconjugated
Mouse IgG3 isotype controlFACS, ELISA, IsoC, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PPV-07 IgG3 unconjugated
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Buffer
- 50 mM tris, 100 mM glycine, pH 7.5
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
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- IgG3
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Target Type
- Antibody
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Background
- Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
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Molecular Weight
- 31.2 kDa
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UniProt
- P01860
Target
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