Catalog No. ABIN2181283
Quick Overview for HEK-293 Cells IgG4 Isotype Control (ABIN2181283)
Target
IgG4
Application
Isotype Control (IsoC)
Isotype
IgG4
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Reactivity
- Human
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Host
- HEK-293 Cells
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Characteristics
- This protein carries no "tag". The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 33-35 kDa under reducing (R) condition, and 50-55 kDa under non-reducing (NR) condition (SDS-PAGE).
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Purity
- >95 % as determined by SDS-PAGE.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Buffer
- 50 mM Tris, 100 mM Glycine, pH 7.5
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
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- IgG4
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Target Type
- Antibody
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Background
- Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
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Molecular Weight
- 25.8 kDa
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UniProt
- P01861
Target
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