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Human IgM Isotype Control

ELISA, WB, IHC, SDS IgM unconjugated

SDS-PAGE of Human IgM Whole Molecule. (Human IgM Isotype Control)

1 image

Catalog No. ABIN5633257

Supplier Product No.: 009-0107-0001

Quick Overview for Human IgM Isotype Control (ABIN5633257)

Target

IgM

Application

ELISA, Western Blotting (WB), Immunohistochemistry (IHC), SDS-PAGE (SDS)

Isotype

IgM

Host
57

46

33

5

2

2

2

2

2

2

2

2

2

2

1
Human

Supplier Product No.

009-0107-0001

Supplier

Rockland

Purpose

Human IgM (myeloma) Whole Molecule

Cross-Reactivity (Details)

Human IgM (myeloma) whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgM (μ chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross-reactivity will occur with anti-Human IgG.

Characteristics

Human immunoglobulin M, Human IgM whole molecule, Human IgM myeloma whole molecule, Hu IgM WM

Purification

Human IgM (myeloma) whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.

Sterility

Sterile filtered
Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC

Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated

Mouse IgM isotype control (PE)
FACS, ELISA Monoclonal 11E10 IgM PE

Rabbit IgM isotype control
IsoC, ELISA, IHC Polyclonal IgM unconjugated

Rat IgM isotype control (PE)
FACS, ELISA Monoclonal NIP-M-2 IgM PE

Mouse IgM isotype control (APC)
FACS, ELISA Monoclonal 11E10 IgM APC

Mouse IgM isotype control
FACS, IsoC, ELISA, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PFR-03 IgM unconjugated

Mouse IgM isotype control (SPRD)
FACS, ELISA Monoclonal 11E10 IgM SPRD

Mouse IgM isotype control (Biotin)
FACS, ELISA Monoclonal 11E10 IgM Biotin

Mouse IgM isotype control (PE-Cy7)
FACS, ELISA Monoclonal 11E10 IgM PE-Cy7

Application Notes

Immunohistochemistry Dilution: User Optimized
Application Note: Human IgM whole molecule has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized

Restrictions

For Research Use only
Format

Liquid

Concentration

1.1 mg/mL

Buffer

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiry Date

12 months
Target

IgM

Target Type

Antibody

Background

Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.

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