Mouse IgM Isotype Control
Quick Overview for Mouse IgM Isotype Control (ABIN964517)
Target
Clonality
Application
Clone
Isotype
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Host
- Mouse
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Supplier Product No.
- 010-001-338
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Supplier
- Rockland
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Purpose
- Mouse IgM Lambda (λ) Isotype Control
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE.
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Characteristics
- Mouse IgM Lambda isotype Control, Mouse IgM Lambda subclass isotype Control
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Purification
- Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.
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Sterility
- Sterile filtered
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Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype control (APC)FACS, ELISA Monoclonal 11E10 IgM APC
Rat IgM isotype control (PE)FACS, ELISA Monoclonal NIP-M-2 IgM PE
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PFR-03 IgM unconjugated
Mouse IgM isotype control (SPRD)FACS, ELISA Monoclonal 11E10 IgM SPRD
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Mouse IgM isotype control (PE-Cy7)FACS, ELISA Monoclonal 11E10 IgM PE-Cy7
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Application Notes
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgM lambda isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- IgM
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Target Type
- Antibody
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Background
- Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.
Target
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