Acetylcholine Assay Kit

Catalog No. ABIN1000286

Quick Overview for Acetylcholine Assay Kit (ABIN1000286)

Target: Acetylcholine (ACH)

Application: Biochemical Assay (BCA)

Sample Type: Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine

Product Details

Characteristics: Use 20 µL samples. Linear detection range: colorimetric assay 10 to 200 µM, fluorimetric assay 0.4 to 10 µM acetylcholine.

Components: Assay Buffer: 10 mL. ACHE Enzyme: 120 µL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 2 mM acetylcholine.

Material not included: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.

Application Details

Application Notes: Assays: acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on acetylcholine metabolism.

Calculation of Results:

Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References

Ahmad, Ramasamy, Jaafar, Majeed, Mani: "Total isoflavones from soybean and tempeh reversed scopolamine-induced amnesia, improved cholinergic activities and reduced neuroinflammation in brain." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 65, pp. 120-8, (2014) (PubMed).

Mani, Ramasamy, Ahmad, Wahab, Jaafar, Kek, Salleh, Majeed: "Effects of the total alkaloidal extract of Murraya koenigii leaf on oxidative stress and cholinergic transmission in aged mice." in: Phytotherapy research : PTR, Vol. 27, Issue 1, pp. 46-53, (2013) (PubMed).

Baitharu, Jain, Deep, Hota, Hota, Prasad, Ilavazhagan: "Withania somnifera root extract ameliorates hypobaric hypoxia induced memory impairment in rats." in: Journal of ethnopharmacology, Vol. 145, Issue 2, pp. 431-41, (2013) (PubMed).

He, Wang, Zhou, Li, Zuo: "Embryonic exposure to benzo(a)pyrene influences neural development and function in rockfish (Sebastiscus marmoratus)." in: Neurotoxicology, Vol. 33, Issue 4, pp. 758-62, (2012) (PubMed).

He, Wang, Li, Wu, Geng, Chen, Zuo: "Exposure of Sebastiscus marmoratus embryos to pyrene results in neurodevelopmental defects and disturbs related mechanisms." in: Aquatic toxicology (Amsterdam, Netherlands), Vol. 116-117, pp. 109-15, (2012) (PubMed).

Chen, Huang, Hu, Yu, Yang, Zhou: "Acute exposure to DE-71: effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae." in: Environmental toxicology and chemistry / SETAC, Vol. 31, Issue 10, pp. 2338-44, (2012) (PubMed).

Target Details

Target: Acetylcholine (ACH)

Alternative Name: Acetylcholine

Target Type: Chemical

Background: Quantitative determination of acetylcholine by colorimetric (570nm) or fluorimetric (530nm/590nm) methods.
Procedure: 30 min.

Acetylcholine is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood. This method provides a simple, direct and high- throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.