IgG ELISA Kit
Quick Overview for IgG ELISA Kit (ABIN1113188)
Target
See all IgG ELISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 20 ng/mL
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Purpose
- Rapid quantification of mouse IgG in cell culture supernatant, follow-up of production process
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Brand
- FastELISA®
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Analytical Method
- Quantitative
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Specificity
- The method enables the detection of all IgG (IgG3 quantification requires a specific standard curve).
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Cross-Reactivity (Details)
- < 1% for Human IgG, < 1.5% for Cow IgG, < 2% for Goat IgG, < 5 % for Swine IgG and < 15 % for Guinea Pig and Rat IgG. The cross reaction with human serum and fetal calf serum is typically below 0.2%.
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Sensitivity
- 6 ng/mL
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Characteristics
- The mouse immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
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IgG ELISA Kit
Reactivity: Rabbit Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP Reactivity: Rabbit
Mouse TrueBlot® Set (with IP beads)WB, IP Reactivity: Mouse
IgG ELISA KitReactivity: Mouse Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Human Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Chicken Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Rat Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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Comment
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One Step ELISA 30 min total time
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Assay Time
- < 1 h
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Plate
- Pre-coated
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Protocol
- A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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Reagent Preparation
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dilution
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Expiry Date
- 12 months
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- IgG
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Alternative Name
- Immunoglobulin G
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Target Type
- Antibody
Target See all IgG ELISA Kits
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