IgG ELISA Kit

Catalog No. ABIN1113190

Cow IgG ELISA Kit Colorimetric assay for quantification of Cow IgG.

Quick Overview for IgG ELISA Kit (ABIN1113190)

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 10-2000 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details IgG ELISA Kit

Minimum Detection Limit: 10 ng/mL

Purpose: Rapid quantification of bovine IgG in batches of purified antibodies, selection of low IgG sera

Brand: FastELISA®

Analytical Method: Quantitative

Specificity: The method enables the detection of bovine IgG.

Characteristics: The bovine immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgG in cell culture supernatants and serums.

Application Details

Comment:

One Step ELISA 45 min total time
IgG contaminations of about 20 ppm

Assay Time: < 1 h

Plate: Pre-coated

Protocol: A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Reagent Preparation:

dilution

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for IgG

Target: IgG

Alternative Name: Immunoglobulin G

Target Type: Antibody