TNFRSF18 ELISA Kit

Catalog No. ABIN1672796

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse TNFRSF18. There is 1 publication available.

Quick Overview for TNFRSF18 ELISA Kit (ABIN1672796)

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Binding Specificity: AA 22-153

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details TNFRSF18 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF18/GITR

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: S22-H153

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S22-H153

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Preferentially expressed in activated T lymphocytes.

Plate: Pre-coated

Protocol: mouse TNFRSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF18 has been precoated onto 96-well plates. Standards(NSO, S22-H153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF18 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFRSF18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF18 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 228, Standard deviation: 10.5, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 1805, Standard deviation: 97.47, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 3208, Standard deviation: 96.24, CV(%): 3,
• Sample 1: n=24, Mean(pg/ml): 324, Standard deviation: 17.82, CV(%): 5.5
• Sample 2: n=24, Mean(pg/ml): 2125, Standard deviation: 144.5, CV(%): 6.8
• Sample 3: n=24, Mean(pg/ml): 3154, Standard deviation: 220.8, CV(%): 7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for TNFRSF18 ELISA Kit (ABIN1672796)

Guiraud, Edwards, Squire, Babbs, Shah, Berg, Chen, Davies: "Identification of serum protein biomarkers for utrophin based DMD therapy." in: Scientific reports, Vol. 7, pp. 43697, (2018) (PubMed).

Target Details for TNFRSF18

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Alternative Name: TNFRSF18

Background:

Protein Function: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway (By similarity). .

Background: Tumor necrosis factor receptor superfamily member 18(TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Synonyms: Tumor necrosis factor receptor superfamily member 18,Glucocorticoid-induced TNFR-related protein,CD357,Tnfrsf18,Gitr,

Full Gene Name: Tumor necrosis factor receptor superfamily member 18

Cellular Localisation: Isoform A: Cell membrane, Single-pass type I membrane protein.

Gene ID: 21936

UniProt: O35714

Pathways: Cancer Immune Checkpoints