Endothelin 1 ELISA Kit

Catalog No. ABIN1672858

Mouse Endothelin 1 ELISA Kit Colorimetric assay for quantification of Mouse Endothelin 1 and has been mentioned in 2+ publications.

Quick Overview for Endothelin 1 ELISA Kit (ABIN1672858)

Target: Endothelin 1 (EDN1)

Binding Specificity: AA 53-73

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9-250 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details Endothelin 1 ELISA Kit

Minimum Detection Limit: 3.9 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Endothelin

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: human peptide
Immunogen sequence: C53-W73

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Predicted Reactivity: Hamster

Sensitivity: <0.5pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: human peptide
Immunogen sequence: C53-W73

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Highest expression in the adult is in lung. Lower levels found in heart, kidney, brain and intestine. In the embryo, expressed in outer and inner pharyngeal arch surfaces. Also expressed in endothelium of dorsal aorta and arch arteries, and in epithelium of pharyngeal pouches. .

Plate: Pre-coated

Protocol: mouse Endothelin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endothelin has been precoated onto 96-well plates. Standards (mouse peptide,C53-W73) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Endothelin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL mouse Endothelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Endothelin standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 22.2, Standard deviation: 0.777, CV(%): 3.5
• Sample 2: n=16, Mean(pg/ml): 85.7, Standard deviation: 4.11, CV(%): 4.8
• Sample 3: n=16, Mean(pg/ml): 144, Standard deviation: 8.21, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 21.4, Standard deviation: 1.46, CV(%): 6.8
• Sample 2: n=24, Mean(pg/ml): 91.5, Standard deviation: 6.68, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 153, Standard deviation: 12.09, CV(%): 7.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Endothelin 1 ELISA Kit (ABIN1672858)

Liu, Zhu, Zhou, Wei, Long, Chen, Ling, Ge, Zhuo: "Endoplasmic reticulum stress promotes amyloid-beta peptides production in RGC-5 cells." in: Cell stress & chaperones, Vol. 19, Issue 6, pp. 827-35, (2014) (PubMed).

Peng, Dai, Ji, Dai: "The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol." in: European journal of pharmacology, Vol. 634, Issue 1-3, pp. 101-6, (2010) (PubMed).

Target Details for Endothelin 1

Target: Endothelin 1 (EDN1)

Alternative Name: EDN1

Background:

Protein Function: Endothelins are endothelium-derived vasoconstrictor peptides.

Background: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

Synonyms: Endothelin-1,ET-1,Preproendothelin-1,PPET1,Edn1,

Full Gene Name: Endothelin-1

Cellular Localisation: Secreted.

Gene ID: 13614

UniProt: P22387

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size