IL-31 ELISA Kit

Catalog No. ABIN1672895

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-31.

Quick Overview for IL-31 ELISA Kit (ABIN1672895)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Binding Specificity: AA 24-163

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details IL-31 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-31

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: T24-C163

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: T24-C163

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse IL-31 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-31 has been precoated onto 96-well plates. Standards(E.coli, T24-C163) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-31 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-31 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-31 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-31 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 387, Standard deviation: 21.3, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 1228, Standard deviation: 78.6, CV(%): 6.4
• Sample 3: n=16, Mean(pg/ml): 2155, Standard deviation: 99.13, CV(%): 4.6,
• Sample 1: n=24, Mean(pg/ml): 453, Standard deviation: 31.26, CV(%): 6.9
• Sample 2: n=24, Mean(pg/ml): 1396, Standard deviation: 100.5, CV(%): 7.2
• Sample 3: n=24, Mean(pg/ml): 2370, Standard deviation: 128, CV(%): 5.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for IL-31

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Alternative Name: IL31

Background:

Protein Function: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. .

Background: Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. The IL31 gene is mapped to chromosome 12q24.31 and the mouse gene maps to a syntenic region on chromosome 5. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma.

Synonyms: Interleukin-31,IL-31,Il31,

Full Gene Name: Interleukin-31

Cellular Localisation: Secreted.

Gene ID: 76399

UniProt: Q6EAL8