(1 reference)Quick Overview for SensoLyte® 390 Cathepsin D Assay Kit (ABIN1882438)
Target
See all Cathepsin D (CTSD) KitsDetection Method
Application
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Brand
- SensoLyte®
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Characteristics
- The SensoLyte® 390 Cathepsin D Activity Assay Kit uses a Mca/Dnp FRET peptide for measurement of enzyme activity. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm.
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Cathepsin D (CTSD) ELISA Kit
CTSD Reactivity: Human Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Human Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Rat Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitHigh Sensitivity CTSD Reactivity: Human Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Cow Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Human AA 21-412 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
SensoLyte® 520 Cathepsin D Assay KitFRET Fluorometric
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Comment
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FRET-based Assay Kit
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Restrictions
- For Research Use only
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Handling Advice
- Protect Components A and B from light and moisture.
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Storage
- -80 °C
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Storage Comment
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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: "Multiple A2E treatments lead to melanization of rod outer segment-challenged ARPE-19 cells." in: Molecular vision, Vol. 20, pp. 285-300, (2014) (PubMed).
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: "Multiple A2E treatments lead to melanization of rod outer segment-challenged ARPE-19 cells." in: Molecular vision, Vol. 20, pp. 285-300, (2014) (PubMed).
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- Cathepsin D (CTSD)
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Alternative Name
- Cathepsin D
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Background
- Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. It is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.
Target
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