(2 references)Quick Overview for SensoLyte® 520 Cathepsin D Assay Kit (ABIN1882439)
Target
See all Cathepsin D (CTSD) KitsDetection Method
Application
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Brand
- SensoLyte®
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Characteristics
- The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL™ 520/HiLyte Fluor™ 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations.
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Cathepsin D (CTSD) ELISA Kit
CTSD Reactivity: Human Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Human Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Rat Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitHigh Sensitivity CTSD Reactivity: Human Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Cow Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin D (CTSD) ELISA KitCTSD Reactivity: Human AA 21-412 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
SensoLyte® 390 Cathepsin D Assay KitFRET Fluorometric
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Comment
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FRET-based Assay Kit
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Restrictions
- For Research Use only
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Handling Advice
- Protect Components A and B from light and moisture.
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Storage
- -80 °C
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Storage Comment
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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: "High-fat diet-induced adipocyte cell death occurs through a cyclophilin D intrinsic signaling pathway independent of adipose tissue inflammation." in: Diabetes, Vol. 60, Issue 8, pp. 2134-43, (2011) (PubMed).
: "Functional heterogeneity of CD11c-positive adipose tissue macrophages in diet-induced obese mice." in: The Journal of biological chemistry, Vol. 285, Issue 20, pp. 15333-45, (2010) (PubMed).
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: "High-fat diet-induced adipocyte cell death occurs through a cyclophilin D intrinsic signaling pathway independent of adipose tissue inflammation." in: Diabetes, Vol. 60, Issue 8, pp. 2134-43, (2011) (PubMed).
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- Cathepsin D (CTSD)
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Alternative Name
- Cathepsin D
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Background
- Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.
Target
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