SensoLyte® 520 Enterokinase Assay Kit

Catalog No. ABIN1882456

Quick Overview for SensoLyte® 520 Enterokinase Assay Kit (ABIN1882456)

Target: TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 Enterokinase Assay Kit employs a novel internally quenched 5- FAM/QXLTM FRET substrate for the detection of enterokinase activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assays are performed in a convenient 96-well microplate format.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light and from moisture.

Storage: -80 °C

Storage Comment: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

Target Details

Target: TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

Alternative Name: Enterokinase

Background: Enterokinase or enteropeptidase is a heterodimeric serine protease produced by cells in the duodenal wall. It converts trypsinogen to trypsin, indirectly activating a number of pancreatic digestive enzymes. Enterokinase is a key enzyme for intestinal digestion of proteins. Its deficiency may cause severe protein malabsorption leading to impaired development and growth. Enterokinase also plays an important role in acute experimental and clinical pancreatitis. The high degree of specificity exhibited by enteropeptidase for cleaving affinity or other tags from recombinant proteins makes it an ideal tool for use in biochemical applications.