SensoLyte® 490 HCV Protease Assay Kit

Catalog No. ABIN1882472

Quick Overview for SensoLyte® 490 HCV Protease Assay Kit (ABIN1882472)

Target: Hepatitis C Virus (HCV)

Reactivity: Hepatitis C Virus (HCV)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® 490 HCV Protease Assay Kit uses an optimized FRET peptide substrate for the continuous measurement of HCV NS3/4a activity. This FRET substrate is cleaved specifically by HCV NS3/4a protease thereby liberating the C-terminal peptide-fluorophore fragment from the proximity quenching effect of the dark quencher, resulting in a more than 10-fold increase in fluorescence. This assay can detect HCV NS3/4a protease activity at 1 pmole.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Components C and D can be stored at room temperature for convenience.

References

Park, Park, Chong: "2-Arylmethylaminomethyl-5,6-dihydroxychromone derivatives with selective anti-HCV activity." in: Bioorganic & medicinal chemistry letters, Vol. 21, Issue 11, pp. 3202-5, (2011) (PubMed).

Peng, Schaefer, Maloof, Skaff, Berical, Belon, Heck, Lin, Frick, Allen, Miziorko, Schreiber, Chung: "Ceestatin, a novel small molecule inhibitor of hepatitis C virus replication, inhibits 3-hydroxy-3-methylglutaryl-coenzyme A synthase." in: The Journal of infectious diseases, Vol. 204, Issue 4, pp. 609-16, (2011) (PubMed).

Yon, Viswanathan, Rossignol, Korba: "Mutations in HCV non-structural genes do not contribute to resistance to nitazoxanide in replicon-containing cells." in: Antiviral research, Vol. 91, Issue 3, pp. 233-40, (2011) (PubMed).

Frick, Ginzburg, Lam: "A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities." in: Methods in molecular biology (Clifton, N.J.), Vol. 587, pp. 223-33, (2010) (PubMed).

Target Details

Target: Hepatitis C Virus (HCV)

Target Type: Virus

Background: HCV protease is identified as an important drug-screening target.