SensoLyte® 520 HIV Protease Assay Kit

Catalog No. ABIN1882481

Quick Overview for SensoLyte® 520 HIV Protease Assay Kit (ABIN1882481)

Target: HIV (Human Immunodeficiency Virus (HIV))

Reactivity: Human Immunodeficiency Virus (HIV)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor™ 488 (fluorophore) and QXL™ 520 (quencher) for continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte Fluor™ 488 is recovered and can be continuously monitored at:
Excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte Fluor™ 488/QXL™ 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

References

Meng, Lai, Munshi, Grobler, McCauley, Zuck, Johnson, Uebele, Hermes, Adam: "Screening of HIV-1 Protease Using a Combination of an Ultra-High-Throughput Fluorescent-Based Assay and RapidFire Mass Spectrometry." in: Journal of biomolecular screening, Vol. 20, Issue 5, pp. 606-15, (2015) (PubMed).

Lu, Sham, Vince: "Design, asymmetric synthesis, and evaluation of pseudosymmetric sulfoximine inhibitors against HIV-1 protease." in: Bioorganic & medicinal chemistry, Vol. 18, Issue 5, pp. 2037-48, (2010) (PubMed).

Target Details

Target: HIV (Human Immunodeficiency Virus (HIV))

Target Type: Virus, Virus