SensoLyte® 520 MMP-14 Assay Kit

Catalog No. ABIN1882505

Quick Overview for SensoLyte® 520 MMP-14 Assay Kit (ABIN1882505)

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: SensoLyte® 520 MMP-14 Assay Kit uses a 5-FAM (fluorophore) and QXL™520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-14, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

References

Mahimkar, Alfonso-Jaume, Cape, Dahiya, Lovett: "Graded activation of the MEK1/MT1-MMP axis determines renal epithelial cell tumor phenotype." in: Carcinogenesis, Vol. 32, Issue 12, pp. 1806-14, (2011) (PubMed).

Sugimoto, Ishibashi, Sawamura, Inoue, Kamioka, Uekita, Ohkawara, Sakamoto, Sakamoto, Okamoto, Takuwa, Kakino, Fujita, Tanaka, Teramoto, Maruyama, Takeishi: "LOX-1-MT1-MMP axis is crucial for RhoA and Rac1 activation induced by oxidized low-density lipoprotein in endothelial cells." in: Cardiovascular research, Vol. 84, Issue 1, pp. 127-36, (2009) (PubMed).

Target Details

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Alternative Name: MMP - 14

Background: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-14 (MT1-MMP) is the membrane bound MMP. When expressed in cells, the catalytic and the hemopexin domains of MMP-14 become exposed on cell surfaces. This enzyme plays an important role in tumor invasion.