(2 references)Quick Overview for SensoLyte® 520 MMP-8 Assay Kit (ABIN1882526)
Target
See all MMP8 KitsDetection Method
Application
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Brand
- SensoLyte®
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Characteristics
- SensoLyte® 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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SensoLyte® 490 MMP-8 Assay Kit
FRET Fluorometric
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Rat AA 21-466 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Human AA 21-467 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum
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Comment
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FRET-based Assay Kit
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Restrictions
- For Research Use only
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Handling Advice
- Protect Components A and B from light.
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Storage
- -20 °C
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Storage Comment
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "Neisseria meningitidis induces brain microvascular endothelial cell detachment from the matrix and cleavage of occludin: a role for MMP-8." in: PLoS pathogens, Vol. 6, Issue 4, pp. e1000874, (2010) (PubMed).
: "Real-time impedance analysis of host cell response to meningococcal infection." in: Journal of microbiological methods, Vol. 84, Issue 1, pp. 101-8, (2010) (PubMed).
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: "Neisseria meningitidis induces brain microvascular endothelial cell detachment from the matrix and cleavage of occludin: a role for MMP-8." in: PLoS pathogens, Vol. 6, Issue 4, pp. e1000874, (2010) (PubMed).
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- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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Alternative Name
- MMP-8
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Background
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-8 (netrophil collagnease) is proposed as a potential anti-cancer drug target.
Target
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