SensoLyte® 520 Rat Renin Assay Kit

Catalog No. ABIN1882548

Quick Overview for SensoLyte® 520 Rat Renin Assay Kit (ABIN1882548)

Target: Renin (REN)

Reactivity: Rat

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 Rat Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL™520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light and moisture.

Storage: -80 °C

Storage Comment: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience

References

Matsushima, Furuuchi, Shirai, Nagai, Yokoyama, Nishida, Hirayama: "Effects of acute and chronic boysenberry intake on blood pressure and endothelial function in spontaneous hypertensive rats." in: Journal of nutritional science and vitaminology, Vol. 60, Issue 1, pp. 43-51, (2014) (PubMed).

Chelko, Schmiedt, Lewis, Lewis, Robertson: "A novel vascular clip design for the reliable induction of 2-kidney, 1-clip hypertension in the rat." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 112, Issue 3, pp. 362-6, (2012) (PubMed).

Shah, Dhar, El Zahraa Mohammed, Habtesion, Davies, Jover-Cobos, Macnaughtan, Sharma, Olde Damink, Mookerjee, Jalan: "Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression." in: Journal of hepatology, Vol. 56, Issue 5, pp. 1047-53, (2012) (PubMed).

Target Details

Target: Renin (REN)

Alternative Name: Renin

Background: Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research.