BMP2 ELISA Kit

Catalog No. ABIN2042361

This Mouse BMP2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse BMP2.

Quick Overview for BMP2 ELISA Kit (ABIN2042361)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details BMP2 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components: 96-well plate pre-coated with anti-BMP-2, Mouse
Lyophilized recombinant BMP-2, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse BMP-2 in bone tissue, cell culture supernates and serum.Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF- ) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP-2

Gene ID: 12156

UniProt: P21274

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation