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BMP2 ELISA Kit

Rat BMP2 ELISA Kit Colorimetric assay for quantification of Rat BMP2.
Catalog No. ABIN2042362

Quick Overview for BMP2 ELISA Kit (ABIN2042362)

Target

See all BMP2 ELISA Kits
BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivity

  • 15
  • 9
  • 8
  • 5
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  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

62.5-4000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    62.5 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 2 pg/mL

    Components

    96-well plate pre-coated with anti-BMP-2, Rat
    Lyophilized recombinant BMP-2, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat BMP-2 in bone tissue, cell culture supernates and serum.Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF- ) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all BMP2 ELISA Kits

    BMP2 (Bone Morphogenetic Protein 2 (BMP2))

    Alternative Name

    BMP-2

    Gene ID

    29373

    UniProt

    P49001

    Pathways

    Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
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