CD86 ELISA Kit

Catalog No. ABIN2042403

This Rat CD86 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat CD86.

Quick Overview for CD86 ELISA Kit (ABIN2042403)

Target: CD86

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details CD86 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-CD86/B7-2, Rat
Lyophilized recombinant CD86/B7-2, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Rat CD86/ B7-2 in cell culture supernates and serum.Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CD86

Target: CD86

Alternative Name: CD86/B7-2

Gene ID: 56822

UniProt: Q62810

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation