Chitinase 3-Like 1/YKL-40 ELISA Kit

Catalog No. ABIN2042405

This Colorimetric ELISA kit is designed for the quantitative measurement of Human not_set.

Quick Overview for Chitinase 3-Like 1/YKL-40 ELISA Kit (ABIN2042405)

Target: Chitinase 3-Like 1/YKL-40

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details

Minimum Detection Limit: 62.5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-Chitinase 3-like 1 /YKL-40, Human
Lyophilized recombinant Chitinase 3-like 1 /YKL-40, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Chitinase 3-like 1 /YKL-40 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kD in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details

Target: Chitinase 3-Like 1/YKL-40

Gene ID: 1116

UniProt: P36222