IL1A ELISA Kit
Quick Overview for IL1A ELISA Kit (ABIN2042511)
Target
See all IL1A ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 4.7 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 0.5 pg/mL
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Components
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96-well plate pre-coated with anti-IL-1 alpha, Human
Lyophilized recombinant IL-1 alpha, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Human IL-1 alpha in cell culture supernates, serum and plasma (heparin, EDTA, citrate.Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant human IL-1 with the molecular mass of 18kD.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- IL1A (Interleukin 1 alpha (IL1A))
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Alternative Name
- IL-1 alpha
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Gene ID
- 3552
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UniProt
- P01583
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Pathways
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
Target See all IL1A ELISA Kits
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