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IL1A ELISA Kit

Rat IL1A ELISA Kit Colorimetric assay for quantification of Rat IL1A.
Catalog No. ABIN2042512

Quick Overview for IL1A ELISA Kit (ABIN2042512)

Target

See all IL1A ELISA Kits
IL1A (Interleukin 1 alpha (IL1A))

Reactivity

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Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

4.9-300 pg/mL

Application

ELISA
  • Minimum Detection Limit

    4.9 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 0.5 pg/mL

    Components

    96-well plate pre-coated with anti-IL-1 alpha, Rat
    Lyophilized recombinant IL-1 alpha, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat IL-1 alpha in cell culture supernates and serum.Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant rat IL-1 with the molecular mass of 18kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IL1A ELISA Kits

    IL1A (Interleukin 1 alpha (IL1A))

    Alternative Name

    IL-1 alpha

    Gene ID

    24493

    UniProt

    P16598

    Pathways

    NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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