IL-10 ELISA Kit

Catalog No. ABIN2042516

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-10.

Quick Overview for IL-10 ELISA Kit (ABIN2042516)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Application: ELISA

Product Details IL-10 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 0.5 pg/mL

Characteristics: Specific detection range: 7.8pg/mL-500pg/mL(body fluids, tissue lysates or cell culture supernates)

Components: 96-well plate pre-coated with anti-IL-10, Human
Lyophilized recombinant IL-10, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human IL-10 in cell culture supernates, serum and plasma (heparin, EDTA, citrate.Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10

Gene ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints