MMP3 ELISA Kit

Catalog No. ABIN2042605

Human MMP3 ELISA Kit Colorimetric assay for quantification of Human MMP3.

Quick Overview for MMP3 ELISA Kit (ABIN2042605)

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details MMP3 ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is cross-reactivates with MMP-10 approximately 2 % , and no detectable cross-reactivity with other MMPs.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-MMP-3, Human
Lyophilized recombinant MMP-3, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human MMP-3 in cell culture supernates, serum and plasma (heparin).Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures.1 MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.2 The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52kD. The detected MMP-3 includes zymogen and active enzyme.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MMP3

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Alternative Name: MMP-3

Gene ID: 4314

UniProt: P08254