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MMP3 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse MMP3.
Catalog No. ABIN2042606

Quick Overview for MMP3 ELISA Kit (ABIN2042606)

Target

See all MMP3 ELISA Kits
MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Reactivity

  • 13
  • 11
  • 6
  • 6
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

6-10000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    6 pg/mL

    Cross-Reactivity (Details)

    There is cross-reactivates with MMP-10 approximately 2 % and no detectable cross-reactivity with other MMPs.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-MMP-3, Mouse
    Lyophilized recombinant MMP-3, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse MMP-3 in cell culture supernates, serum and plasma (heparin).Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all MMP3 ELISA Kits

    MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

    Alternative Name

    MMP-3

    Gene ID

    17392

    UniProt

    P28862
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