BMP2 ELISA Kit

Catalog No. ABIN2043011

This Colorimetric ELISA kit is designed for the quantitative measurement of Cow BMP2.

Quick Overview for BMP2 ELISA Kit (ABIN2043011)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details BMP2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.107 ng/mL

Application Details

Plate: Pre-coated

Protocol: Bone Morphogenetic Protein 2 (BMP2) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 2 (BMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Bone Morphogenetic Protein 2 (BMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation