CENPE ELISA Kit

Catalog No. ABIN2043113

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CENPE.

Quick Overview for CENPE ELISA Kit (ABIN2043113)

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details CENPE ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.124 ng/mL

Application Details

Plate: Pre-coated

Protocol: Centromere Protein E (CENPE) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Centromere Protein E (CENPE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Centromere Protein E (CENPE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Centromere Protein E (CENPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Centromere Protein E (CENPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CENPE

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Alternative Name: Centromere Protein E (CENPE)

Pathways: M Phase, Maintenance of Protein Location