CD163 ELISA Kit

Catalog No. ABIN2043171

This Rat CD163 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat CD163.

Quick Overview for CD163 ELISA Kit (ABIN2043171)

Target: CD163

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details CD163 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cluster Of Differentiation (CD163) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation (CD163). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation (CD163). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation (CD163), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cluster Of Differentiation (CD163) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CD163

Target: CD163

Alternative Name: Cluster Of Differentiation (CD163)