DEFB103A ELISA Kit

Catalog No. ABIN2043301

Product Details DEFB103A ELISA Kit

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 12.7 pg/mL

Application Details

Plate: Pre-coated

Protocol: Defensin Beta 103B (DEFB103B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103B (DEFB103B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103B (DEFB103B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103B (DEFB103B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 103B (DEFB103B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DEFB103A

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Alternative Name: Defensin beta 103B (DEFB103B) (DEFB103A Products)

Pathways: Production of Molecular Mediator of Immune Response