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Albumin ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Albumin.
Catalog No. ABIN2043647

Quick Overview for Albumin ELISA Kit (ABIN2043647)

Target

See all Albumin (ALB) ELISA Kits
Albumin (ALB)

Reactivity

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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.15-10 ng/mL

Application

ELISA
  • Minimum Detection Limit

    0.15 ng/mL

    Sensitivity

    < 0.048 ng/mL
  • Plate

    Pre-coated

    Protocol

    High Sensitivity Albumin (ALB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Albumin (ALB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Albumin (ALB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Albumin (ALB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Albumin (ALB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all Albumin (ALB) ELISA Kits

    Albumin (ALB)

    Pathways

    Lipid Metabolism
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