IgA ELISA Kit
Quick Overview for IgA ELISA Kit (ABIN2043727)
Target
See all IgA ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.93 ng/mL
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Sensitivity
- < 0.42 ng/mL
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IgA ELISA Kit
Reactivity: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgA ELISA KitReactivity: Human Colorimetric Sandwich ELISA 0.14 ng/mL - 100 ng/mL Plasma, Saliva, Serum
IgA ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
IgA ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
IgA ELISA KitReactivity: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
IgA ELISA KitReactivity: Sheep Colorimetric Competition ELISA 3.75 μg/mL - 60 μg/mL Plasma, Serum
IgA ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 31.25 ng/mL - 1000 ng/mL Plasma, Serum, Urine
IgA ELISA KitReactivity: Human Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Urine
IgA ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgA ELISA KitReactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
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Plate
- Pre-coated
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Protocol
- Immunoglobulin A (IgA) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin A (IgA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin A (IgA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Immunoglobulin A (IgA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- IgA
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Alternative Name
- Immunoglobulin A (IgA)
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Target Type
- Antibody
Target See all IgA ELISA Kits
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