IGFBP2 ELISA Kit

Catalog No. ABIN2043757

Chicken IGFBP2 ELISA Kit Colorimetric assay for quantification of Chicken IGFBP2.

Quick Overview for IGFBP2 ELISA Kit (ABIN2043757)

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Reactivity: Chicken

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details IGFBP2 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Brand: BioAssay™

Sensitivity: < 7.5 pg/mL

Application Details

Plate: Pre-coated

Protocol: Insulin Like Growth Factor Binding Protein 2 (IGFBP2) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 2 (IGFBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 2 (IGFBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 2 (IGFBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Like Growth Factor Binding Protein 2 (IGFBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IGFBP2

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Alternative Name: Insulin Like Growth Factor Binding Protein 2 (IGFBP2)

Pathways: Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation