PDX1 ELISA Kit

Catalog No. ABIN2043763

Product Details PDX1 ELISA Kit

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Insulin Promoter Factor 1 (IPF) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Promoter Factor 1 (IPF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Promoter Factor 1 (IPF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Promoter Factor 1 (IPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Promoter Factor 1 (IPF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: Insulin Promoter Factor 1 (IPF) (PDX1 Products)

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location